User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/21

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September 21, 2016 : Fluorescence III

Objective

  1. Collect fluorescence data for lysozyme at pH 6
  2. Make stock solutions for UV-Vis on 9.27.2016

Description

Fluoresence

  1. Lysozyme solution was made according to the measurements found in data below
  2. Lysozyme solution was run through florescence for 3 hours with data taken every 5 minutes

Stock Solutions for 9.27.2016

Solutions were made in glass test tubes, covered with foil, and placed in an oven for 4 hours at 80 C.

Data

Fluorescence

  1. Solution Measurements
    1. Lysosome Stock Solution
      1. concentration = 225 μM
    2. Gold Stock Solution
      1. concentration = 3.73 mM
    3. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 6
      1. 223 μL AuCl3
      2. 3 μL 1M HCl
      3. 167 μL Lysozyme
      4. 2607 μL Water

SUMMARY DATA AND FIGURES

Summary Data at 15 minute increments
Time(minutes) Maximum Emission Integrated Intensity
0 24.520899 3712.15643
15 56.486824 7231.99931
30 49.689208 6161.526628
45 48.143968 5814.874805
60 51.558296 6106.685527
75 54.792335 6387.85191
90 58.186188 6671.018707
105 61.668483 7022.28452
120 65.251046 7297.185504
135 67.23012 7504.95303
150 68.580282 7665.567058
165 70.167388 7820.376345
180 72.220234 8021.858662




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