User:Cosette Brianna Taggart/Notebook/Experimental/2016/08/31: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 224: Line 224:
|}
|}
*'''[[User:Matt Hartings|Matt Hartings]] 14:04, 1 September 2016 (EDT)''': Your sample prep didn't work. See my lab notebook page [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/01|here]] for a description of why and how I remade your samples.
*'''[[User:Matt Hartings|Matt Hartings]] 14:04, 1 September 2016 (EDT)''': Your sample prep didn't work. See my lab notebook page [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/01|here]] for a description of why and how I remade your samples.
*'''[[User:Matt Hartings|Matt Hartings]] 12:52, 2 September 2016 (EDT)''': I have taken the complementary data for a sample with no gold. Please see data [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/02|here]]




Revision as of 09:52, 2 September 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

August 31, 2016

Objectives

  1. Collect fluorescence data for lysozyme at pH 4
  2. Make stock solutions for UV-Vis on 9.06.2016

Description

Fluoresence

  1. Lysozyme solution was made according to the measurements found in data below
  2. Lysozyme solution was run through florescence for 3 hours with data taken every 5 minutes

Stock Solutions for 9.06.2016

Solutions were made in glass test tubes, covered with foil, and placed in an oven for 4 hours at 80 C.

Data

Fluorescence

  1. Solution Measurements
    1. Lysosome Stock Solution
      1. 8.88 mg of lysozyme with water in 10.0 mL volumetric flask
      2. MW = 14307 g/mol
      3. concentration = 62.1 μM
    2. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 4
      1. 225 μL AuCl3
      2. 300 μL 1M HCl
      3. 604 μL Lysozyme
      4. 1871 μL Water

ADD DATA POINTS AND GRAPHS

Solution Prep

Lysozyme UVVis solution measurement 0 mM fructose

pH HCl (μL) NaOHc (μL) NaOHd (μL) AuCl3 (μL) Fructose (μL) Lysozyme (μL) Water (μL)
4 500 0 0 300 0 252 3949
5 50 0 0 300 0 252 4399
6 5 0 0 300 0 252 4444
7 0 0 0 300 0 252 4449
8 0 0 5 300 0 252 4444
9 0 0 50 300 0 252 4399
10 0 0 500 300 0 252 3949
11 0 5 0 300 0 252 4444
12 0 50 0 300 0 252 4399

Lysozyme UVVis solution measurement .0625 mM fructose

pH HCl (μL) NaOHc (μL) NaOHd (μL) AuCl3 (μL) Fructose (μL) Lysozyme (μL) Water (μL)
4 500 0 0 300 24 252 3925
5 50 0 0 300 24 252 4375
6 5 0 0 300 24 252 4420
7 0 0 0 300 24 252 4425
8 0 0 5 300 24 252 4420
9 0 0 50 300 24 252 4375
10 0 0 500 300 24 252 3925
11 0 5 0 300 24 252 4420
12 0 50 0 300 24 252 4375
  • Matt Hartings 14:04, 1 September 2016 (EDT): Your sample prep didn't work. See my lab notebook page here for a description of why and how I remade your samples.
  • Matt Hartings 12:52, 2 September 2016 (EDT): I have taken the complementary data for a sample with no gold. Please see data here



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Cosette_Brianna_Taggart/Notebook/Experimental/2016/08/31&oldid=948171"