User:Corey Bear/Notebook/09 July 2014 Identifying and Studying Bacteria: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(Identifying and Studying Bacteria In Hay Infusion Culture)
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Intent:''' The intent of this experiment was to understand the characteristics of bacteria to observe antibiotic resistance, and to properly identify DNA sequences presence from PCR in later processing. 


‘’’Intent:’’’ The intent of this experiment was to understand the characteristics of bacteria to observe antibiotic resistance, and to properly identify DNA sequences presence from the PCR in later processing.
'''Overview:''' During this lab, the serial dilution results were observed. Two agar plates were chosen from both the nutrient positive and tetracycline plates, and extracted utilizing sterilized tools to conduct a Gram Stain Procedure. Additionally, each of the four agar plates that were observed also had content extracted to three 16s DNA sequencing tubes for the process of performing a PCR.  


‘’’Overview:’’’ During this lab, the serial dilution results were observed. Two agar plates where chosen from both the nutrient positive and tetracycline plates, and extracted utilizing sterilized tools to conduct a Gram Stain Procedure. Additionally, each of the four agar plates that where observed also had content extracted to three 16s DNA sequencing tubes for the process of performing a PCR.  
'''Observations:''' This experiment is controlled under optimal conditions suitable for normal bacteria. Because of this, the probability of archaea presence in the samples is unlikely, due to the extreme conditions in which they thrive. Additional growth from the current bacteria has as smell of rotten food. Continued growth and bacteria proliferation is anticipated to continue due to environmental changes and ample amounts of nutrients. This continued growth is anticipated until either the environment does not suit the organisms’ fitness or nutrient depletion. The agar plates which had tetracycline showed signs of fewer colonies. Under observation, the antibiotic plates had large presence of orange growth in the colonies. This indicated that bacteria, in the presence of antibiotics, are not as prevalent, and therefore temporarily affected by the antibiotics. The overall effect present with tetracycline hinders bacterial growth, but has limited affect on fungi growth. From observation, the orange bacteria present in the agars has either produced a resistance to the antibiotic or is not a bacteria. “Tetracycline works by interfering with the ability of bacteria to produce proteins that are essential to them. Without these proteins the bacteria cannot grow, multiply and increase in numbers. Tetracycline therefore stops the spread of the infection and the remaining bacteria are killed by the immune system or eventually die.” (Netdoctor) Because of this, tetracycline treats bacteria infections of the urinary tract, Syphillis, Gonorrhoea, and other types of bacterial conditions.


‘’’Observations:’’’ This experiment is controlled under optimal conditions suitable for normal bacteria. Because of this, the likelihood of archaea present in the samples is unlikely, due to the extreme conditions in which they thrive. Additional grow from the current bacteria has as smell of rotten food. Continued growth and bacteria proliferation continued due to to environmental changes and ample amounts of nutrients. This continued growth is anticipated until either the environments does not suit the organisms’ fitness or nutrient depletion.
During observations under a compound microscope, the following information were observed, cited in (Table 2).  


[[Table 1]]: https://drive.google.com/file/d/0B0_IEyKChqDxVEdBeFJQWFhlbWc/edit?usp=sharing


Additional observations:
[[Table 2:]] https://drive.google.com/file/d/0B0_IEyKChqDxQ1JZMF92WjdFa0U/edit?usp=sharing


 
Reference
Intent: This process is a follow on step from the original transect experiment underway. The intent of this process is to analyze the original transect derived from a predefined ecosystem located on American University. 100 microliters of content from the Hay Infusion Culture previously constructed were added to the first of four test tubes, then sequentially transferring 100 microliters to each test tube, from the one previous (this creates a dilution factor of: 10-2, 10-4, 10-6, 10-8), and then added to agars for a plating serial dilution.
(Netdoctor) Tetracycline tablets: How does it work? Retrieved from http://www.netdoctor.co.uk/sex-and-relationships/medicines/tetracycline-tablets.html.
Overview: 100 microliters of content from the Hay Infusion Culture previously constructed were added to the first of four test tubes, filled with 10 mililiters of sterile broth, then sequentially transferring 100 microliters to each test tube. Each tube had100 microliters added from the previous (exception of the first), which has a dilution factor of: 10E-2, 10E-4, 10E-6, and 10E-8. Two agar plats were prepared for each test tube, one which was sterile and one with tertracycline. Each plate had 100 micro liters added, and then stirred with a sterile glass rode. This created a dilution factor of: 10E-3, 10E-5, 10E-7, and 10E-9 Upon completion, the plates were placed near the window for further processing.
Observations: Samples of the Hay Infusion Culture were examined under a 40x compound microscope to identify potential organisms present prior to further analysis. The links below are a few organisms that were seen during this observation.
Chilomonas: https://drive.google.com/file/d/0B0_IEyKChqDxLTFBQ2l4NHRCR2M/edit?usp=sharing
Euglena: https://drive.google.com/file/d/0B0_IEyKChqDxY0UyakVXLUZjTTA/edit?usp=sharing
Paramecium Bursaria: https://drive.google.com/file/d/0B0_IEyKChqDxSzF4TmpfTVpHLUU/edit?usp=sharing
Peranema Sp: https://drive.google.com/file/d/0B0_IEyKChqDxTkc2RW9TYVg4OTg/edit?usp=sharing
Volvox: https://drive.google.com/file/d/0B0_IEyKChqDxQ2trSW1zUXo0Y3M/edit?usp=sharing
Serial Dilution Diagram: https://drive.google.com/file/d/0B0_IEyKChqDxMEpNa1lKUmlHUzQ/edit?usp=sharing
Additional observations: at first glance, the Hay Infusion Culture separated by density. This was seen through the segregation between the moldy top and thinker sediment bottom, with murky water in-between; an odor of decaying wetlands and moldy milk was also present. Moreover, organisms seen in the above links were motile and very present by the thousands in a single screening through a microscope. (sizes and descriptions of these organisms can be seen in the links provided.) Subsequently, these extant organisms fall within both the prokaryotic and Eukaryotic domains.
‘’’Predictions:’’’ If observations continued for additional months, the continued grow would permit under optimal conditions; pending nutrients and environmental factors remaining constant. This may yield an increase in the current colonies present in the Hay Infusion Culture.
 
==Notes==
* Place some notes here for visitors
 
* Example: This project is currently on hold until further notice.
 
 
|-
|colspan="2" style="background-color: #F2F2F2;"|
{{LnNotebookRecentChanges2}}
|}
 
__NOTOC__

Latest revision as of 20:50, 9 July 2014

Intent: The intent of this experiment was to understand the characteristics of bacteria to observe antibiotic resistance, and to properly identify DNA sequences presence from PCR in later processing.

Overview: During this lab, the serial dilution results were observed. Two agar plates were chosen from both the nutrient positive and tetracycline plates, and extracted utilizing sterilized tools to conduct a Gram Stain Procedure. Additionally, each of the four agar plates that were observed also had content extracted to three 16s DNA sequencing tubes for the process of performing a PCR.

Observations: This experiment is controlled under optimal conditions suitable for normal bacteria. Because of this, the probability of archaea presence in the samples is unlikely, due to the extreme conditions in which they thrive. Additional growth from the current bacteria has as smell of rotten food. Continued growth and bacteria proliferation is anticipated to continue due to environmental changes and ample amounts of nutrients. This continued growth is anticipated until either the environment does not suit the organisms’ fitness or nutrient depletion. The agar plates which had tetracycline showed signs of fewer colonies. Under observation, the antibiotic plates had large presence of orange growth in the colonies. This indicated that bacteria, in the presence of antibiotics, are not as prevalent, and therefore temporarily affected by the antibiotics. The overall effect present with tetracycline hinders bacterial growth, but has limited affect on fungi growth. From observation, the orange bacteria present in the agars has either produced a resistance to the antibiotic or is not a bacteria. “Tetracycline works by interfering with the ability of bacteria to produce proteins that are essential to them. Without these proteins the bacteria cannot grow, multiply and increase in numbers. Tetracycline therefore stops the spread of the infection and the remaining bacteria are killed by the immune system or eventually die.” (Netdoctor) Because of this, tetracycline treats bacteria infections of the urinary tract, Syphillis, Gonorrhoea, and other types of bacterial conditions.

During observations under a compound microscope, the following information were observed, cited in (Table 2).

Table 1: https://drive.google.com/file/d/0B0_IEyKChqDxVEdBeFJQWFhlbWc/edit?usp=sharing

Table 2: https://drive.google.com/file/d/0B0_IEyKChqDxQ1JZMF92WjdFa0U/edit?usp=sharing

Reference (Netdoctor) Tetracycline tablets: How does it work? Retrieved from http://www.netdoctor.co.uk/sex-and-relationships/medicines/tetracycline-tablets.html.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Corey_Bear/Notebook/09_July_2014_Identifying_and_Studying_Bacteria&oldid=801264"