User:Christine Doan/Notebook/Synthetic Biology/2014/03/21: Difference between revisions
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*** 37C HybB promoter ceases expression | *** 37C HybB promoter ceases expression | ||
** Future direction: exchange the HybB promoter for the CspA promoter (BioBrick BBa_K328001) to produce anti-freeze protein that prevents ice crystal formation in the cell | ** Future direction: exchange the HybB promoter for the CspA promoter (BioBrick BBa_K328001) to produce anti-freeze protein that prevents ice crystal formation in the cell | ||
** Parts used: | |||
* AHUT-China: Shining Purifier (2011) | * AHUT-China: Shining Purifier (2011) | ||
** Goal: design a wastewater cleansing system to efficiently absorb pollutants and transform them to light | ** Goal: design a wastewater cleansing system to efficiently absorb pollutants and transform them to light | ||
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** Current method is anaerobic ammonium oxidation bacteria (anammox), which converts fomite to nitrogen, but it's time-consuming and oxygen-sensitive | ** Current method is anaerobic ammonium oxidation bacteria (anammox), which converts fomite to nitrogen, but it's time-consuming and oxygen-sensitive | ||
** Synthesize a biofilm in 4-6 days | ** Synthesize a biofilm in 4-6 days | ||
** | ** Signal factor (a) prompts bacteria to stretch out flagellum, (b) release mucus, and (c) promote reproduction | ||
*** Increased surface area = decreased release of signal factors | |||
*** No interaction among the signal factors | |||
** Parts used: | |||
*** Fluorescent protein device (BBa_K1196016) | |||
*** Nitrite reductase device (BBa_K1196018) | |||
*** Hydrazine synthetase device (BBa_K1196019) | |||
*** Hydrazine oxidoreductase device (BBa_K1196020) | |||
*** Formate dehydrogenase device (BBa_K1196021) | |||
*** NADH:ubiquonone reductase device (BBa_K1196022) | |||
*** Cytochrome c reducase device (BBa_K1196023) | |||
* Calgary: Detect and Destroy: Building FRED and OSCAR (2012) | |||
** Convert toxins into useable hydrocarbons | |||
** Tailings ponds contain byproducts of the bitumen extraction process of oil sands | |||
** FRED: Functional Robust Electrochemical Detector | |||
*** Multiple output biosensor | |||
** OSCAR: Optimized System for Carboxylic Acid Remediation | |||
*** Designed to target naphthenic acids (carboxylic acid-containing compounds), catechol, and nitrogen and sulfur containing heterocyclic compounds) | |||
** Parts used: | |||
*** PetroBrick (BBa_K590025) | |||
*** xylE (BBa_J33204) | |||
Revision as of 12:22, 25 March 2014
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Relevant BioBricks
Previous iGEM Nitrate Biosensor Projects
Biosensor Database [2] |