User:Christine Doan/Notebook/Identifying Kinase Substrates of PfCK2/2014/02/24
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CK2
1. Transform
2. Select colonies, grow in 5ml LB + Amp ON 3. Inoculate 2.5ml into 250ml LB, grow to OD600 = 0.5-0.6 4. Induce with 0.5mM IPTG, incubate 20hrs at 20C
Sub 4
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