User:Chia-Hung Tai/Notebook/SBB11/Entry Base

Entry 1: 2/15/11

Prepared for PCR for pBca1766-sbb1141 and pBjh1601KC-sbb1116 -pBca1766-sbb1141 --Phusion is needed since the template is a plasmid DNA --Instead of adding 3.3 uM 10x of the Expand Buffer, make it 5x by adding 6.6 uM --Set up the PCR tubes following the protocol

-pBjh1601KC-sbb1116 --Dilute the oligos --Add 363 uL of water (10 times the concentration) to cht002R --Add 268 uL of water (10 times the concentration) to cht002F --Take 1 uL of the mixture for each oligo and add 9 microL of water to get 10 uL

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