User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/27

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Objective

  • Finish Kinetic Data and calculate average
  • Begin UV runs with calculated inhibitor

Description

  • Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
    • The following concentrations were used.
Concentration [Ado] (uM) Velocity (nm/sec) 1/[Ado] (1/uM) 1/velocity (sec/nm) ' (-1)/Km -0.022385428
10.52 3.70166E-05 0.095057034 27014.92537 Km 44.67191792
12.34 5.13812E-05 0.081037277 19462.36559 1/Vmax 2662.131337
14.93 6.0221E-05 0.066979236 16605.50459 Vmax 0.000375639
18.87 7.45856E-05 0.052994171 13407.40741
25.64 8.67403E-05 0.03900156 11528.66242
40 9.94475E-05 0.025 10055.55556
  • 10mM Quercetin stock was prepared.
  • Cuvettes with the following solutions were prepared and analyzed.
    • Today, we only had time to complete the first two.
[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Volume Inhibitor Stock solution added [Inhibitor Stock Solution] (uM) [Final Inhibitor] (uM) Total Volume (mL)
0.5 0.02 0.00333 52.63157895 0.6 10.52631579 1.78 0.6 65 13 3
0.5 0.02 0.00333 61.72839506 0.6 12.34567901 1.78 0.6 65 13 3
0.5 0.02 0.00333 74.62686567 0.6 14.92537313 1.78 0.6 65 13 3
0.5 0.02 0.00333 94.33962264 0.6 18.86792453 1.78 0.6 65 13 3
0.5 0.02 0.00333 128.2051282 0.6 25.64102564 1.78 0.6 65 13 3
0.5 0.02 0.00333 200 0.6 40 1.78 0.6 65 13 3

Data

  • The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.

  • Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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