|
|
(3 intermediate revisions by 2 users not shown) |
Line 6: |
Line 6: |
| | colspan="2"| | | | colspan="2"| |
| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| ==Entry title==
| |
|
| |
|
|
| |
|
|
| |
|
| ==Objective==
| |
| *Finish Kinetic Data and calculate average
| |
| *Begin UV runs with calculated inhibitor
| |
|
| |
|
| ==Description==
| |
| *Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
| |
| **The following concentrations were used.
| |
| {| {{table}}
| |
| | align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
| |
| | align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
| |
| | align="center" style="background:#f0f0f0;"|''''''
| |
| | align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
| |
| | align="center" style="background:#f0f0f0;"|'''-0.022385428'''
| |
| |-
| |
| | 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
| |
| |-
| |
| | 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
| |
| |-
| |
| | 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
| |
| |-
| |
| | 18.87||7.45856E-05||0.052994171||13407.40741||||||
| |
| |-
| |
| | 25.64||8.67403E-05||0.03900156||11528.66242||||||
| |
| |-
| |
| | 40||9.94475E-05||0.025||10055.55556||||||
| |
| |-
| |
| |
| |
| |}
| |
|
| |
|
| *10mM Quercetin stock was prepared.
| |
| *Cuvettes with the following solutions were prepared and analyzed.
| |
| **Today, we only had time to complete the first two.
| |
|
| |
|
| {| {{table}}
| |
| | align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Volume Inhibitor Stock solution added'''
| |
| | align="center" style="background:#f0f0f0;"|'''[Inhibitor Stock Solution] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''[Final Inhibitor] (uM)'''
| |
| | align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
| |
| |-
| |
| | 0.5||0.02||0.00333||52.63157895||0.6||10.52631579||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||61.72839506||0.6||12.34567901||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||74.62686567||0.6||14.92537313||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||94.33962264||0.6||18.86792453||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||128.2051282||0.6||25.64102564||1.78||0.6||65||13||3
| |
| |-
| |
| | 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
| |
| |-
| |
| |
| |
| |}
| |
|
| |
|
| ==Data==
| |
| *The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.
| |
| [[Image:3_5data.JPG]]
| |
|
| |
| *Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.
| |
|
| |
|
| ==Notes== | | ==Notes== |