User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12: Difference between revisions

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==Concentration Tables==
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
| 5000||200||800||1000||1
|-
| 5000||150||850||750||1
|-
| 5000||100||900||500||1
|-
| 5000||40||960||200||1
|-
| 5000||16||984||80||1
|-
| 5000||6.4||993.6||32||1
|}
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
|-
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
|-
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
|-
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
|-
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
|-
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
|}


==Data==
==Data==

Revision as of 14:50, 8 May 2013

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Entry title

Data

Run#1

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.000116022
150 0.000192818
100 0.000139227
40 5.55556E-06
16 6.68508E-05
6.4 0.000039
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 8619.047619
0.006666667 5186.246418
0.01 7182.539683
0.025 180000
0.0625 14958.67769
0.15625 25641.02564


Run#2

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.00021989
150 0.000147514
100 0.000144751
40 0.00010884
16 8.12155E-05
6.4 3.8674E-05
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 4547.738693
0.006666667 6779.026217
0.01 6908.396947
0.025 9187.817259
0.0625 12312.92517
0.15625 25857.14286

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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