User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12

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*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 +
**Dilution Tables are located below
==Concentration Tables==
==Concentration Tables==

Revision as of 13:22, 15 February 2013

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Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • Dilution Tables are located below

Concentration Tables

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
500020080010003
50001508507503
50001009005003
5000409602003
500016984803
50006.4993.6323

Notes

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