User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12

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***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]]
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==Data==
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==Concentration Tables==
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* Add data and results here...
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
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| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
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|-
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| 5000||200||800||1000||3
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|-
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| 5000||150||850||750||3
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|-
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| 5000||100||900||500||3
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|-
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| 5000||40||960||200||3
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|-
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| 5000||16||984||80||3
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|-
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| 5000||6.4||993.6||32||3
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|}
==Notes==
==Notes==

Revision as of 13:21, 15 February 2013

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Entry title

Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • 150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
    • 200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
    • This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
      • {| border="1" cellpadding="4" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;"
[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
500020080010001
50001508507501
50001009005001
5000409602001
500016984801
50006.4993.6321
    • This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
      • Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png

Concentration Tables

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
500020080010003
50001508507503
50001009005003
5000409602003
500016984803
50006.4993.6323

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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