User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12: Difference between revisions

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==Description==
==Description==
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*The original Adenosine stock solution was diluted using the table below:
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
*The assay components were mixed using the table below:
**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
**200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
***{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
| 5000||200||800||1000||1
|-
| 5000||150||850||750||1
|-
| 5000||100||900||500||1
|-
| 5000||40||960||200||1
|-
| 5000||16||984||80||1
|-
| 5000||6.4||993.6||32||1
|}
**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]]


==Data==
==Data==

Revision as of 10:17, 15 February 2013

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Objective

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Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • 150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
    • 200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
    • This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
      • {| border="1" cellpadding="4" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;"
 [Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
5000 200 800 1000 1
5000 150 850 750 1
5000 100 900 500 1
5000 40 960 200 1
5000 16 984 80 1
5000 6.4 993.6 32 1
    • This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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