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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
Learn how to maintain an OpenWetWare Notebook.


==Description==
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
**Dilution Tables are located below


==Concentration Tables==
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
| 5000||200||800||1000||1
|-
| 5000||150||850||750||1
|-
| 5000||100||900||500||1
|-
| 5000||40||960||200||1
|-
| 5000||16||984||80||1
|-
| 5000||6.4||993.6||32||1
|}
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
|-
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
|-
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
|-
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
|-
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
|-
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
|}
==Data==
Run#1
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
|-
| 200||0.000116022
|-
| 150||0.000192818
|-
| 100||0.000139227
|-
| 40||5.55556E-06
|-
| 16||6.68508E-05
|-
| 6.4||0.000039
|}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
|-
| 0.005||8619.047619
|-
| 0.006666667||5186.246418
|-
| 0.01||7182.539683
|-
| 0.025||180000
|-
| 0.0625||14958.67769
|-
| 0.15625||25641.02564
|}




Run#2


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
|-
| 200||0.00021989
|-
| 150||0.000147514
|-
| 100||0.000144751
|-
| 40||0.00010884
|-
| 16||8.12155E-05
|-
| 6.4||3.8674E-05
|}


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
|-
| 0.005||4547.738693
|-
| 0.006666667||6779.026217
|-
| 0.01||6908.396947
|-
| 0.025||9187.817259
|-
| 0.0625||12312.92517
|-
| 0.15625||25857.14286
|}


==Notes==
==Notes==

Latest revision as of 22:27, 26 September 2017

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