User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12: Difference between revisions

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==Objective==
Learn how to maintain an OpenWetWare Notebook.


==Description==
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
**200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
***{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
| 5000||200||800||1000||1
|-
| 5000||150||850||750||1
|-
| 5000||100||900||500||1
|-
| 5000||40||960||200||1
|-
| 5000||16||984||80||1
|-
| 5000||6.4||993.6||32||1
|}
**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]]


==Concentration Tables==
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
| 5000||200||800||1000||3
|-
| 5000||150||850||750||3
|-
| 5000||100||900||500||3
|-
| 5000||40||960||200||3
|-
| 5000||16||984||80||3
|-
| 5000||6.4||993.6||32||3
|}


==Notes==
==Notes==

Revision as of 14:50, 8 May 2013

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