User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12

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*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
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**150 uM adenosine stock solution was used; (stock) 150uL 5mM stock + 850 uL buffer =750 uM ->use .6mL new stock for ADA assay
 
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**200 uM adenosine stock solution was used: (stock) 200 uL 5mM stock+800 uL buffer = 1000uM ->use 0.6mL new stock for ADA assay
 
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**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
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***{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 
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|-
 
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| 5000||200||800||1000||1
 
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|-
 
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| 5000||150||850||750||1
 
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|-
 
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| 5000||100||900||500||1
 
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|-
 
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| 5000||40||960||200||1
 
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|-
 
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| 5000||16||984||80||1
 
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|-
 
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| 5000||6.4||993.6||32||1
 
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|}
 
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**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 
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***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]]
 
==Concentration Tables==
==Concentration Tables==

Revision as of 13:22, 15 February 2013

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Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:

Concentration Tables

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
500020080010003
50001508507503
50001009005003
5000409602003
500016984803
50006.4993.6323

Notes

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