User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/02/12

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(Objective)
Current revision (16:50, 8 May 2013) (view source)
(Data)
 
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==Objective==
 
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Repeat ADA Assay 2 times for additional data
 
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==Description==
 
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*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
 
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*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
 
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**Dilution Tables are located below
 
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==Concentration Tables==
 
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This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 
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|-
 
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| 5000||200||800||1000||1
 
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|-
 
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| 5000||150||850||750||1
 
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|-
 
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| 5000||100||900||500||1
 
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|-
 
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| 5000||40||960||200||1
 
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|-
 
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| 5000||16||984||80||1
 
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|-
 
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| 5000||6.4||993.6||32||1
 
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|}
 
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This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 
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|-
 
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| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||750||0.6||150||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||500||0.6||100||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||200||0.6||40||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||80||0.6||16||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
 
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|}
 
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==Data==
 
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Run#1
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 
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|-
 
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| 200||0.000116022
 
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|-
 
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| 150||0.000192818
 
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|-
 
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| 100||0.000139227
 
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|-
 
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| 40||5.55556E-06
 
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|-
 
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| 16||6.68508E-05
 
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|-
 
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| 6.4||0.000039
 
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|}
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 
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|-
 
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| 0.005||8619.047619
 
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|-
 
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| 0.006666667||5186.246418
 
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|-
 
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| 0.01||7182.539683
 
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|-
 
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| 0.025||180000
 
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|-
 
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| 0.0625||14958.67769
 
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|-
 
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| 0.15625||25641.02564
 
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|}
 
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Run#2
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
 
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|-
 
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| 200||0.00021989
 
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|-
 
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| 150||0.000147514
 
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|-
 
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| 100||0.000144751
 
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|-
 
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| 40||0.00010884
 
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|-
 
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| 16||8.12155E-05
 
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|-
 
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| 6.4||3.8674E-05
 
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|}
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
 
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|-
 
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| 0.005||4547.738693
 
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|-
 
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| 0.006666667||6779.026217
 
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|-
 
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| 0.01||6908.396947
 
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|-
 
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| 0.025||9187.817259
 
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|-
 
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| 0.0625||12312.92517
 
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|-
 
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| 0.15625||25857.14286
 
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|}
 
==Notes==
==Notes==

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