User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/11/09: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2011/11/09 Entry for User:Catherine_Koenigsknecht/Notebook/Experimental_Biological_Chemistry)
 
Line 14: Line 14:


==Description==
==Description==
#  
#Place three test tubes in a water bath at 80C
*test tube 1: 50 uL BSA 50 uL HAuCl water
*test tube 2: 50 uL HAuCl 100 uL BSA water
*test tube 3: PCR tube equal parts BSA and HAuCl
*After two hours start to cool bath using ice
 
#Place three test tubes in heat block at 80C
*test tube 1: 50 uL BSA 50 uL HAuCl water
*test tube 2: 50 uL HAuCl 100 uL BSA water
*test tube 3: PCR tube equal parts BSA and HAuCl
 
#Place test tube in PCR
*run at 80C for two hours then decrease 2C every 2 min until the sample reaches room temperature
 
#Run microwave reaction with 400 uL BSA 400 uL HAuCl and water to the top (about 8 mL) at 300 W 80C 30 min
#Allow full cool down time


==Data==
==Data==

Revision as of 11:21, 9 November 2011

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  1. Place three test tubes in a water bath at 80C
  • test tube 1: 50 uL BSA 50 uL HAuCl water
  • test tube 2: 50 uL HAuCl 100 uL BSA water
  • test tube 3: PCR tube equal parts BSA and HAuCl
  • After two hours start to cool bath using ice
  1. Place three test tubes in heat block at 80C
  • test tube 1: 50 uL BSA 50 uL HAuCl water
  • test tube 2: 50 uL HAuCl 100 uL BSA water
  • test tube 3: PCR tube equal parts BSA and HAuCl
  1. Place test tube in PCR
  • run at 80C for two hours then decrease 2C every 2 min until the sample reaches room temperature
  1. Run microwave reaction with 400 uL BSA 400 uL HAuCl and water to the top (about 8 mL) at 300 W 80C 30 min
  2. Allow full cool down time

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Catherine_Koenigsknecht/Notebook/Experimental_Biological_Chemistry/2011/11/09&oldid=565466"