User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/10/05: Difference between revisions
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==Description== | ==Description== | ||
# | The calculated concentration of the MBP protein on 9/13/11 was 10.714μM | ||
# First, the 10.714μM MBP was diluted by mixing 1397μL of it with 1603μL of 50mM Tris buffer, pH 7.55. This gave a final concentration of 4.989μM. | |||
#* The intensities of this was taken from 310nm-500nm when excited at 290nm. | |||
# Then, 300μL of the 4.989μM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 0.4989μM. | |||
#* The intensities of this was taken from 310nm-500nm when excited at 290nm. | |||
# Then, 300μL of the 0.4989μM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 49.89nM. | |||
#* The intensities of this was taken from 310nm-500nm when excited at 290nm. | |||
# Then, 300μL of the 49.89nM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 4.989nM. | |||
#* The intensities of this was taken from 310nm-500nm when excited at 290nm. | |||
# Then, 300μL of the 4.989nM MBP was diluted with 2700μL of the same Tris buffer for a final concentration of 0.4989nM. | |||
#* The intensities of this was taken from 310nm-500nm when excited at 290nm. | |||
==Data== | ==Data== |
Revision as of 09:32, 26 December 2011
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Entry titleObjectiveTake fluorescence spectra of the maltose-binding protein (MBP). DescriptionThe calculated concentration of the MBP protein on 9/13/11 was 10.714μM
Data
NotesThis area is for any observations or conclusions that you would like to note.
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