User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/10/04: Difference between revisions
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==Data== | ==Data== | ||
First, standards of Ru(bpy)(phen) in acetonitrile were made. The stock solution of Ru(bpy)(phen) is 360μM: | |||
{| border="1" | |||
|- | |||
!Standard !!Amount of Ru(bpy)(phen) !!Amount of acetonitrile !!Final Concentration in Cuvette | |||
|- | |||
!Standard 1 | |||
|60 μL ||2940 μL ||7.2 μM | |||
|- | |||
!Standard 2 | |||
|120 μL ||2880 μL ||14.4μM | |||
|- | |||
!Standard 3 | |||
|180μL ||2820μL ||21.6μM | |||
|- | |||
!Standard 4 | |||
|240μL ||2760μL ||28.8μM | |||
|- | |||
!Standard 5 | |||
|300μL ||2700μL ||36μM | |||
|- | |||
!Standard 6 | |||
|600μL ||2400μL ||72μM | |||
|- | |||
!Standard 7 | |||
|900μL ||2100μL ||108μM | |||
|} | |||
The intensities taken were corrected with the intensities of acetonitrile, and the spectrum of intensities was graphed: | |||
[[Image:Ru_intensity_100411.png|600px]] | |||
The curves were integrated in Excel and the following areas under the curves were found: | |||
{| border="1" | |||
|- | |||
!Concentration (μM) !!Integrated Intensity | |||
|- | |||
! 7.2 | |||
|682.1927 | |||
|- | |||
!14.4 | |||
|886.9565 | |||
|- | |||
!21.6 | |||
|1091.243 | |||
|- | |||
!28.8 | |||
|1339.258 | |||
|- | |||
!36 | |||
|1436.954 | |||
|- | |||
!72 | |||
|1767.737 | |||
|- | |||
!108 | |||
|1877.2334 | |||
|} | |||
These integrated intensity were plotted against the concentrations to see the sensitivity of the instrument: | |||
[[Image:Integrals_100411.png|600px]] | |||
The calculated integrated intensities for all data collected from the class was graphed in the same way: | |||
[[Image:Class_fluor_data_102711.png|600px]] | |||
==Notes== | ==Notes== |
Revision as of 09:17, 26 December 2011
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Entry titleObjective
DescriptionDataFirst, standards of Ru(bpy)(phen) in acetonitrile were made. The stock solution of Ru(bpy)(phen) is 360μM:
The intensities taken were corrected with the intensities of acetonitrile, and the spectrum of intensities was graphed: The curves were integrated in Excel and the following areas under the curves were found:
These integrated intensity were plotted against the concentrations to see the sensitivity of the instrument:
NotesThis area is for any observations or conclusions that you would like to note.
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