User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/09/21: Difference between revisions
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==Description== | ==Description== | ||
# | # Run 50mL of the supernatent from yesterday over the column at 1mL/min. Collect 10mL fractions. | ||
# Wash the column with 70mL of column buffer at 1mL/min. Collect 10mL fractions. | |||
# Elute the column with 25mL of 10mM maltose in column buffer at 1mL/min. Collect 5mL fractions. | |||
# Run 150mL of the supernatent from yesterday over the column at 1mL/min. Collect 10mL fractions. | |||
# Wash with 75mL of column buffer at 1mL/min. Collect 10mL fractions. | |||
# Elute the column with 75mL of 10mM maltose in column buffer at 1mL/min. Collect 5mL fractions. | |||
==Data== | ==Data== |
Revision as of 10:06, 4 October 2011
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Entry titleContinue to purify using the column. ObjectiveLearn how to maintain an OpenWetWare Notebook. Description
Data
NotesThis area is for any observations or conclusions that you would like to note.
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