User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/09/20: Difference between revisions
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==Description== | ==Description== | ||
# | Extraction | ||
# The | #Defrost cells that were stored at -20°C. | ||
# | #The cells were sonicated for 15 seconds at a time with approximately one minute on ice between sonications. In total the cells were sonicated for 90 seconds. | ||
#The cells were centrifuged at 9000g for 30 minutes at 4°C. | |||
Purification | |||
#The column was poured. Amylose resin was used. The column volume was 15mL. | |||
#120mL of the column buffer was run over the column at 2mL/min. | |||
==Data== | ==Data== |
Revision as of 10:02, 4 October 2011
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Entry titleObjectiveExtracting protein from the cell cultures. DescriptionExtraction
Purification
Data
NotesThis area is for any observations or conclusions that you would like to note.
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