Flourescence
Procedure
- Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
- Au 2.93mM
- (0.25mM)(3.0mL)=(XmL)(2.93mM)
- XmL= .256mL= 256uL Au
- Myoglobin 133uM
- (12.5uM)(3.0mL)=(XmL)(133uM)
- X=0.282mL= 282uL Myoglobin
- 3uL 1mM NaOH
- Water
- Volume of water= 3000uL- 256uL-282uL-3uL= 2459uL
- Absorbance measurements were taken and saved every 3 minutes for 3 hours
- The instrument was set at 80 degrees Celsius
- The scan range parameters were start at 310nm and ending at 540nm
- The emission and exit slit were set at 10nm
Solution for UV-Vis Preparation
Stock Concentrations:
- Myoglobin 70uM for 0.75mM Fructose solutions, 67.76uM for 1.25mM Fructose solution
- Silver 25.8mM
- Fructose 33.0mM
0.75mM Fructose
pH
|
μL of 1 mM HNO3stock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AgNO33stock
|
μL of Fructosestock
|
μL of Myoglobinstock
|
μL of Water
|
4 |
500 |
0 |
0 |
48 |
113 |
886 |
3451
|
5 |
50 |
0 |
0 |
48 |
113 |
886 |
3901
|
6 |
5 |
0 |
0 |
48 |
113 |
886 |
3946
|
7 |
0 |
0 |
0 |
48 |
113 |
886 |
3951
|
8 |
0 |
5 |
0 |
48 |
113 |
886 |
3946
|
9 |
0 |
50 |
0 |
48 |
113 |
886 |
3901
|
10 |
0 |
500 |
0 |
48 |
113 |
886 |
3451
|
11 |
0 |
0 |
5 |
48 |
113 |
886 |
3946
|
12 |
0 |
0 |
50 |
48 |
113 |
886 |
3901
|
1.25 mM Fructose
pH
|
μL of 1 mM HNO3stock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AgNO33stock
|
μL of Fructosestock
|
μL of Myoglobinstock
|
μL of Water
|
4 |
500 |
0 |
0 |
48 |
189 |
922 |
3339
|
5 |
50 |
0 |
0 |
48 |
189 |
922 |
3789
|
6 |
5 |
0 |
0 |
48 |
189 |
922 |
3834
|
7 |
0 |
0 |
0 |
48 |
189 |
922 |
3839
|
8 |
0 |
5 |
0 |
48 |
189 |
922 |
3834
|
9 |
0 |
50 |
0 |
48 |
189 |
922 |
3787
|
10 |
0 |
500 |
0 |
48 |
189 |
922 |
3339
|
11 |
0 |
0 |
5 |
48 |
189 |
922 |
3834
|
12 |
0 |
0 |
50 |
48 |
189 |
922 |
3789
|
|