User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/10/19: Difference between revisions
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# As with gold, it is at pH 11 and pH 12 that unique spectra are seen. | # As with gold, it is at pH 11 and pH 12 that unique spectra are seen. | ||
## The solutions are more clear at pH 11 and pH 12 than for the other solutions. | ## The solutions are more clear at pH 11 and pH 12 than for the other solutions. | ||
## As fructose concentration increases, so does the cloudiness of the solutions | ## As fructose concentration increases, so does the cloudiness of the solutions for pH 11 and pH 12 | ||
## pH was also more clear for all fructose concentrations. | ## pH 12 was also more clear for all fructose concentrations. | ||
==Solutions== | |||
#All of the solutions for both concentrations of fructose were cloudy. Below are photos of pH 11 and 12 respectively, going in order from 0mM Fructose- 0.25mM fructose | |||
Ag NP's, myoglobin, pH11: 0mM, .0625mM, .125mM, .25mM Fructose | |||
[[Image:AgNP 11pH 0-0.25mM fructose.jpg|600px]] | |||
Ag NP's, myoglobin, pH12: 0mM, .0625mM, .125mM, .25mM Fructose | |||
[[Image:AgNP pH12 fructose 0mM-.25mM.jpg|600px]] | |||
==Data== | |||
# Graphs for .125mM Fructose | |||
Absorbance v. Wavelength | |||
[[Image:.125mM fructose AgNP myoglobin.png|600px]] | |||
Absorbance at 272nm | |||
[[Image:Absorbance at 272nm .125mM fructose myoglobin.png|600px]] | |||
Absorbance at 400nm | |||
[[Image:Absorbance at 400nm AgNP.png|600px]] | |||
#Graphs for 0.25mM Fructose | |||
Absorbance v. Wavelength | |||
[[Image:Absorbance v wavelength AgNP .25mM fructose.png|600px]] | |||
Absorbance at 396nm | |||
[[Image:Absorbance at 396nm AgNP .25mM fructose.png|600px]] | |||
Absorbance at 284nm | |||
[[Image:Absorbance at 284nm AgNP .25mM fructose.png|600px]] | |||
==Future Experiments To Do== | |||
# When making UV-Vis next time, also remake 0mM fructose and 0.0625mM fructose pH 9 and pH 5 samples. | |||
# Control experiments: myoglobin, gold, fructose, silver | |||
[[User:Matt Hartings/Notebook/AU Biomaterials Design Lab]] | |||
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Latest revision as of 02:06, 27 September 2017
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UV-VisProcedure
Observations
Solutions
Ag NP's, myoglobin, pH11: 0mM, .0625mM, .125mM, .25mM Fructose Ag NP's, myoglobin, pH12: 0mM, .0625mM, .125mM, .25mM Fructose Data
Absorbance v. Wavelength Absorbance at 272nm Absorbance at 400nm
Absorbance v. Wavelength Absorbance at 396nm Absorbance at 284nm Future Experiments To Do
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