User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/10/18

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Flourescence

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.95mM
      1. (0.25mM)(3.0mL)=(XmL)(3.95mM)
      2. XmL= .190mL= 190uL Au
    2. Myoglobin 133uM
      1. (12.5uM)(3.0mL)=(XmL)(133uM)
      2. X=0.282mL= 282uL Myoglobin
    3. Water
      1. Volume of water= 3000uL- 190uL-282uL= 2528uL
  2. Absorbance measurements were taken and saved every 3 minutes for 3 hours
  3. The instrument was set at 80 degrees Celsius
  4. The scan range parameters were start at 310nm and ending at 540nm
  5. The emission and exit slit were set at 10nm



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