User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/10/18: Difference between revisions
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== | ==Flourescence== | ||
==Procedure== | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | |||
##Au 3.95mM | |||
###(0.25mM)(3.0mL)=(XmL)(3.95mM) | |||
###XmL= .190mL= 190uL Au | |||
##Myoglobin 133uM | |||
###(12.5uM)(3.0mL)=(XmL)(133uM) | |||
###X=0.282mL= 282uL Myoglobin | |||
##Water | |||
###Volume of water= 3000uL- 190uL-282uL= 2528uL | |||
#Absorbance measurements were taken and saved every 3 minutes for 3 hours | |||
#The instrument was set at 80 degrees Celsius | |||
#The scan range parameters were start at 310nm and ending at 540nm | |||
#The emission and exit slit were set at 10nm | |||
Scans AuNP Myoglobin pH7 | |||
[[Image:Scans of myoglobin AuNP pH7.png|600px]] | |||
Wavelength of Myoglobin AuNp's at pH7 | |||
[[Image:Wavelength myoglobin AuNP pH7.png|600px]] | |||
Maximum Intensity of Myoglobin AuNp's at pH7 | |||
[[Image:Max intensity Myo AuNP pH7.png|600px]] | |||
Integrated Intensity of Myoglobins AuNp's at pH7 | |||
[[Image:Integrated intensity AuNP myo pH7.png|600px]] | |||
==Observations== | |||
#The maximum wavelength shifted around 2 hours 10 minutes to the left peak. This left peak is normally seen around 2 hours 30 minutes but is not normally higher than the original peak. | |||
[[User:Matt Hartings/Notebook/AU Biomaterials Design Lab]] | |||
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Latest revision as of 02:05, 27 September 2017
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FlourescenceProcedure
Scans AuNP Myoglobin pH7 Wavelength of Myoglobin AuNp's at pH7 Maximum Intensity of Myoglobin AuNp's at pH7 Integrated Intensity of Myoglobins AuNp's at pH7 Observations
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