User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/09/21: Difference between revisions

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==Entry title==
==Ocean Optics 9/21/16==
* Insert content here...
* The pH 5 sample from [[User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/09/13 |9/13/2016]] was redone with the correct concentration of myoglobin.


==Procedure==
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 0.1mM HCl 3mL total
##Au 3.73mM
###(0.25mM)(3.0mL)=(XmL)(3.73mM)
###XmL= .201mL= 201uL Au
##Myoglobin 160uM
###(12.5uM)(3.0mL)=(XmL)(160uM)
###X=0.234mL= 234uL Myoglobin
##HCl 0.1mM
###(3.0mL)(0.01mM)=(1.0mM)(XmL)
###XmL=0.03mL= 30uL HCl
##Water
###Volume of water= 3000uL- 201uL-234uL-30uL= 2535uL
##Scans were taken at 80oC every 2 minutes for 3 hours
==Results==
#Scans
Absorbance Scan at pH5
[[Image:Ocean optics pH5.png|600px]]
Wavelength Scan at pH5
[[Image:Ocean optics wavelength ph5.png|600px]]
[[User:Matt Hartings/Notebook/AU Biomaterials Design Lab]]


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Latest revision as of 02:01, 27 September 2017

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Ocean Optics 9/21/16

  • The pH 5 sample from 9/13/2016 was redone with the correct concentration of myoglobin.

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 0.1mM HCl 3mL total
    1. Au 3.73mM
      1. (0.25mM)(3.0mL)=(XmL)(3.73mM)
      2. XmL= .201mL= 201uL Au
    2. Myoglobin 160uM
      1. (12.5uM)(3.0mL)=(XmL)(160uM)
      2. X=0.234mL= 234uL Myoglobin
    3. HCl 0.1mM
      1. (3.0mL)(0.01mM)=(1.0mM)(XmL)
      2. XmL=0.03mL= 30uL HCl
    4. Water
      1. Volume of water= 3000uL- 201uL-234uL-30uL= 2535uL
    5. Scans were taken at 80oC every 2 minutes for 3 hours

Results

  1. Scans

Absorbance Scan at pH5

Wavelength Scan at pH5


User:Matt Hartings/Notebook/AU Biomaterials Design Lab


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