User:Cassandra M Barrett/Notebook/Open Chromatin/2015/11/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==pJET Sequencing== | ||
Purpose: to verify the inserts from the pJET LCR reactions | |||
Methods: | |||
Reconstitute pJET1.2 primers to 100uM. Dilute to 10uM working stocks | |||
Set up for each sequencing reaction 6uL total, 1uL of primer, 150-200ng DNA, to 6uL with water | |||
For SOLS submission: | |||
https://sols.asu.edu/resources/core-laboratories | |||
Fill in form 1, bring to Life Sciences Tower E, room s17 | |||
Latest revision as of 01:21, 27 September 2017
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pJET SequencingPurpose: to verify the inserts from the pJET LCR reactions Methods: Reconstitute pJET1.2 primers to 100uM. Dilute to 10uM working stocks Set up for each sequencing reaction 6uL total, 1uL of primer, 150-200ng DNA, to 6uL with water For SOLS submission: https://sols.asu.edu/resources/core-laboratories Fill in form 1, bring to Life Sciences Tower E, room s17
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