User:Cassandra M Barrett/Notebook/Open Chromatin/2015/10/07: Difference between revisions
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*Expected results for MV10_Gal4DB_mCh_ATF2: 1814bp, 5459bp | *Expected results for MV10_Gal4DB_mCh_ATF2: 1814bp, 5459bp | ||
All lanes appeared to be around 5kb like the sequence verified MV10 control. We can tell that the LCR didn't work and that the MV10 isolated from the + control is most likely the right thing. | |||
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Revision as of 11:29, 7 October 2015
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LCR Plasmid DigestPurpose: to check for any properly constructed plasmids via digesting sample and running it on a gel to check proper fragment size Methods: Using PstI FD, set up 11 digests: LCR colonies 1-8, purified MV10 from yesterday, sequence verified MV10 used for transformation, water Create the following mastermix (X12)
Aliquot 18uL of mastermix and add 2uL of appropriate DNA. Incubate at 37C for 15min. Run samples on 1% agrose gel for 45min at 110V Results:
All lanes appeared to be around 5kb like the sequence verified MV10 control. We can tell that the LCR didn't work and that the MV10 isolated from the + control is most likely the right thing. | |
