User:CarolineAjo-Franklin

Hallo!
I'm a more recent addition to the Silver Lab in the area of yeast synthetic biology. We (Dirk, Ira, and myself) have designed and are in the process of constructing a cell-cycle counter in S. cerevisiae. Our goal is to produce an in vivo device which will identity cells which have divided 0, 1, or 2 times through expression of fluorescent reporters. Our motivation is three-fold: 1)the cell-cycle counter and related devices would provide vastly improved tools for aging research in S. cerevisiae, 2)construction of a functional counter should reveal quantitative attributes of robust genetic networks, and 3) the counter would be the first example of a synthetic device in eukaryotic cells which takes advantage of sub-cellular compartmentalization - the major feature that distinguishes eukaryotes.