User:Carly M. Montanero/Notebook/CHEM-571/2013/10/16: Difference between revisions
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:* Just before 1 minute, add 1 mL of 60:1 Au:HRP solution. | :* Just before 1 minute, add 1 mL of 60:1 Au:HRP solution. | ||
'''BSA-AuNP Synthesis''' | |||
# Add 1.00 mL of 2.33 mM gold solution (HAuCL<sub>4</sub>) to a 10 mL volumetric flask. | |||
# Add 1.73 mL BSA (90x more gold than BSA) to the volumetric flask. | |||
# Dilute the solution up to 10 mL with deionized water. | |||
# Transfer the solution to a test tube and cap with aluminum foil. | |||
# Heat in the oven at 80°C for four hours. | |||
# Transfer the solution to a plastic falcon tube. | |||
'''Remaking the Solution of Gold and HRP''' | |||
# For 60:1 Au:HRP solution, 2.56 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water. | |||
# The 10 mL solution of gold and HRP was placed into a test tube. | |||
# The test tube was placed in the oven at 80°C for four hours. | |||
==Figures== | |||
[[Image:Cmj_HRPKineticsUV-Vis_10.16.2013.png ]] | |||
*Note: Spectra overlap for a total elapsed time of 19 minutes. | |||
[[Image:Cmj_HRPKinetics_520overtime_10.16.2013.png]] | |||
==Notes== | ==Notes== | ||
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* The stock concentration of luminol was 1.46 mM. | * The stock concentration of luminol was 1.46 mM. | ||
* The stock concentration of hydrogen peroxide was 44.29 mM. | * The stock concentration of hydrogen peroxide was 44.29 mM. | ||
* The stock concentration of gold (HAuCL<sub>4</sub>)was 2.33 mM. | |||
* The stock concentration of BSA was 15 μM. | |||
* The stock solution of gold was 2.94 mM. | |||
* The stock solution of HRP was 16.27 μM. | |||
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Revision as of 05:08, 29 October 2013
Biomaterials Design Lab: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo test the activity of our HRP-NPs for the catalytic conversion of luminol. ProcedureCreating the Luminol-H2O2 Solution
Enzyme Kinetics Measurement
BSA-AuNP Synthesis
Remaking the Solution of Gold and HRP
Figures
Notes
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