User:Carly M. Montanero/Notebook/CHEM-571/2013/10/15

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Objective

To make HRP-AuNPs for enzymatic studies.

Procedure

Creating the Solutions of Gold and HRP

  1. For 30:1 Au:HRP solution, 5.10 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
  2. For 60:1 Au:HRP solution, 2.56 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
  3. For 90:1 Au:HRP solution, 1.71 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
  4. For 120:1 Au:HRP solution, 1.28 mL HRP stock and 850.34 μL were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.

Notes

  • The stock solution of gold was 2.94 mM.
  • The stock solution of HRP was 16.27 μM.


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