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 Biomaterials Design Lab: Fall 2013
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Objective
To make HRP-AuNPs for enzymatic studies.
Procedure
Creating the Solutions of Gold and HRP
- For 30:1 Au:HRP solution, 5.10 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
- For 60:1 Au:HRP solution, 2.56 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
- For 90:1 Au:HRP solution, 1.71 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
- For 120:1 Au:HRP solution, 1.28 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water.
Synthesis of HRP-AuNPs
- The 10 mL solutions of gold and HRP were placed into four different test tubes.
- The test tubes were placed in the oven at 80°C for approximately 3 hours.
Notes
- The stock solution of gold was 2.94 mM.
- The stock solution of HRP was 16.27 μM.
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Biomaterials Design Lab: Fall 2013
