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Cameron Neylon
School of Chemistry
University of Southampton
Southampton
SO17 1BJ, UK |
ISIS Neutron Facility
STFC Rutherford Appleton Laboratory
Chilton, Didcot
OX11 0QX, UK
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| I am a Lecturer in Chemical Biology at the School of Chemistry at the University of Southampton with a joint appointment at the ISIS Pulsed Neutron Source, Rutherford Appleton Laboratory, near Oxford. The group at Southampton works in the areas of protein-ligand binding [1, 2, 3] and directed evolution [4]with a focus on methods development for high throughput analysis and protein labeling[5]. We have recently developed methodology for the immobilisation of proteins on solid supports as well as the site selective fluorescent labelling of proteins. I am also involved in the 4G Basic Technology project which is developing encoded microparticles for use in high throughput assays. The project has demonstrated the feasibility of encoding manufactured particles using minature diffraction gratings, the ability to carry out multistep synthesis on these particles [6], as well as performing multiplexed immunoassays and DNA hybridisation. We have also analysed the ability of high throughput DNA sequencing [7] to deliver useful information and developed new tools for designing hybridisation probes for DNA analysis [8].
At the Rutherford Laboratory we are developing and promoting neutron and X-ray scattering techniques for applications in the biological sciences. Scattering techniques provide a means of obtaining low resolution information on biomolecules in solution. It is particularly useful in determining the structure of biomolecular complexes in solution, especially where high resolution structural data is available on the individual components of the complex.
The combination of high throughput methodology and working on two sites has led to a developing programme in e-science particularly in the area of electronic lab notebooks. In collaboration with the e-science group at Southampton and Jeremy Frey we are developing and testing an electronic notebook based on a blog. This has the potential to provide a very flexible framework for recording and analysing data but is currently at an early stage of development.
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Research Interests
- Methods for selective protein labelling
- Directed evolution
- High throughput methods for genotype and phenotype analysis
- Small angle X-ray and Neutron scattering
- Electronic lab notebooks and automatic data capture
References
- Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, and Dixon NE. A molecular mousetrap determines polarity of termination of DNA replication in E. coli. Cell. 2006 Jun 30;125(7):1309-19. DOI:10.1016/j.cell.2006.04.040 | PubMed ID:16814717 | HubMed [cell06]
- Neylon C, Kralicek AV, Hill TM, and Dixon NE. Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. Microbiol Mol Biol Rev. 2005 Sep;69(3):501-26. DOI:10.1128/MMBR.69.3.501-526.2005 | PubMed ID:16148308 | HubMed [mmbr05]
- Neylon C, Brown SE, Kralicek AV, Miles CS, Love CA, and Dixon NE. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry. 2000 Oct 3;39(39):11989-99. DOI:10.1021/bi001174w | PubMed ID:11009613 | HubMed [biochem00]
- Neylon C. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution. Nucleic Acids Res. 2004;32(4):1448-59. DOI:10.1093/nar/gkh315 | PubMed ID:14990750 | HubMed [nar04]
- Wood RJ, Pascoe DD, Brown ZK, Medlicott EM, Kriek M, Neylon C, and Roach PL. Optimized conjugation of a fluorescent label to proteins via intein-mediated activation and ligation. Bioconjug Chem. 2004 Mar-Apr;15(2):366-72. DOI:10.1021/bc0341728 | PubMed ID:15025533 | HubMed [bioconjchem04]
- Cavalli G, Banu S, Ranasinghe RT, Broder GR, Martins HF, Neylon C, Morgan H, Bradley M, and Roach PL. Multistep synthesis on SU-8: combining microfabrication and solid-phase chemistry on a single material. J Comb Chem. 2007 May-Jun;9(3):462-72. DOI:10.1021/cc060079p | PubMed ID:17417911 | HubMed [jcombchem07]
- Whiteford N, Haslam N, Weber G, Prügel-Bennett A, Essex JW, Roach PL, Bradley M, and Neylon C. An analysis of the feasibility of short read sequencing. Nucleic Acids Res. 2005 Nov 7;33(19):e171. DOI:10.1093/nar/gni170 | PubMed ID:16275781 | HubMed [nar06]
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Weber G, Haslam N, Whiteford N, Prugel-Bennett A, Essex JW, and Neylon C. Thermal equivalence of DNA duplexes without calculation of melting temperature. Nature Physics, 2(1), 55-59, doi:10.1038/nphys189
All Medline abstracts: PubMed | HubMed
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