User:Cameron Neylon

Cameron Neylon School of Chemistry University of Southampton Southampton United Kingdom SO17 1BJ C.Neylon@rl.ac.uk

I am a Lecturer in Chemical Biology at the School of Chemistry at the University of Southampton with a joint appointment at the ISIS Pulsed Neutron Source, Rutherford Appleton Laboratory, near Oxford. The group at Southampton works in the areas of protein-ligand binding and directed evolution with a focus on methods development for high throughput analysis and protein labeling. At the Rutherford Lab we are developing and promoting neutron and X-ray scattering techniques for applications in the biological sciences.

The combination of high throughput methodology and working on two sites has led to a developing programme in e-science particularly in the area of electronic lab notebooks.

Research Interests

• Methods for selective protein labelling
• Directed evolution
• High throughput methods for genotype and phenotype analysis
• Small angle X-ray and Neutron scattering
• Electronic lab notebooks and automatic data capture

Publications

1. Cavalli G, Banu S, Ranasinghe RT, Broder GR, Martins HF, Neylon C, Morgan H, Bradley M, and Roach PL. Multistep synthesis on SU-8: combining microfabrication and solid-phase chemistry on a single material. J Comb Chem. 2007 May-Jun;9(3):462-72. DOI:10.1021/cc060079p |
2. Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, and Dixon NE. A molecular mousetrap determines polarity of termination of DNA replication in E. coli. Cell. 2006 Jun 30;125(7):1309-19. DOI:10.1016/j.cell.2006.04.040 |
3. Whiteford N, Haslam N, Weber G, Prügel-Bennett A, Essex JW, Roach PL, Bradley M, and Neylon C. An analysis of the feasibility of short read sequencing. Nucleic Acids Res. 2005 Nov 7;33(19):e171. DOI:10.1093/nar/gni170 |

4. Weber G, Haslam N, Whiteford N, Prugel-Bennett A, Essex JW, and Neylon C. Thermal equivalence of DNA duplexes without calculation of melting temperature. Nature Physics, 2(1), 55-59, doi:10.1038/nphys189

5. Neylon C, Kralicek AV, Hill TM, and Dixon NE. Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. Microbiol Mol Biol Rev. 2005 Sep;69(3):501-26. DOI:10.1128/MMBR.69.3.501-526.2005 |
6. Wood RJ, Pascoe DD, Brown ZK, Medlicott EM, Kriek M, Neylon C, and Roach PL. Optimized conjugation of a fluorescent label to proteins via intein-mediated activation and ligation. Bioconjug Chem. 2004 Mar-Apr;15(2):366-72. DOI:10.1021/bc0341728 |
7. Neylon C. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution. Nucleic Acids Res. 2004;32(4):1448-59. DOI:10.1093/nar/gkh315 |
8. Neylon C, Brown SE, Kralicek AV, Miles CS, Love CA, and Dixon NE. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry. 2000 Oct 3;39(39):11989-99. DOI:10.1021/bi001174w |