User:Brigette D. Black/Notebook/Brigettes Notebook/2009/06/01: Difference between revisions
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== | ==Optics & Microscopy Review== | ||
* Quick summery of some concepts in optics | * Quick summery of some concepts in optics | ||
*The entirety of this entry is lifted from wikipedia, follow the links for the full articles | *The entirety of this entry is lifted from wikipedia, follow the links for the full articles |
Revision as of 20:59, 1 June 2009
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Optics & Microscopy Review
Köhler IlluminationKöhler Illumination [1] is the proper alignment of incident or illuminating light, this technique yields optimal illumination for entire field of view. Process
DIC MicroscopyDifferential interference contrast (DIC)[2] microscopy is a technique designed to enhance contrast in a sample. This technique can gain information about the optical density of the sample and can produce an image that gives the appearance of a three-dimensional physical relief. The final image appears three dimensional with light and dark shadows. This method is very useful as it allows for observing the the entire shape of the object. The picture on the side shows the light path of a reflected DIC microscope (taken from [3]) Process:
Numerical ApertureThe numerical aperture[4] of an optic tells the maximum diameter of a beam for which it is functional. It is defined by
where n is the index of refraction of the medium in which the optic is placed (air, oil, etc) and θ is the half-angle of the maximum cone of light that can enter/exit the optic. |