User:Bohao Liu/Notebook/Research/2012/10/20: Difference between revisions

From OpenWetWare
< User:Bohao Liu‎ | Notebook‎ | Research‎ | 2012‎ | 10
Jump to navigationJump to search
(Autocreate 2012/10/20 Entry for User:Bohao_Liu/Notebook/Research)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Title==
*Materials
**LFM
**LFM+1M sorbitol
**Flow Cells
**Ethanol (70%)
**H20
**conA
**Run fluorescene first as a control or do you think we are ok ???


 
*Methods (general)
===Smaller Title===
**Inoculate cells t = -38 hrs
*Stuff
**Set back cells t = -14 hrs
#Numbered stuff
**Set up flow cell
***plasma clean, holes, etc.
***wash flow cell with Eth and water
***add ConA for at least 5 mins
***measure OD of cells
***add cells and  allow to sit for 5 mins
**Measurement Parameters
***Times
****210
****180
****150
****120
****100
****90
****80
****60
****30
****10
*Methods (specific)
**Set1: 210 and 180
**Set2: 150 and 120
**run Set1Wt, Set1Mutant, Set2Wt, Set2 Mutant on one day
**Set3: 100 and 90 and 80
**Set4: 60 and 30 and 10
**run Set3Wt, Set3Mutant, Set4Wt, Set4Mutant on another day
**Never sleep
**5 oscillations per time ie for 60 secs run 11 minutes, start and end with off
**Wait 20 minutes to allow cells to reset in between different times of the same set
**I need to go through the timing, but i think i need 3 days to get through each mutant


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 12:45, 26 June 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
  • Materials
    • LFM
    • LFM+1M sorbitol
    • Flow Cells
    • Ethanol (70%)
    • H20
    • conA
    • Run fluorescene first as a control or do you think we are ok ???
  • Methods (general)
    • Inoculate cells t = -38 hrs
    • Set back cells t = -14 hrs
    • Set up flow cell
      • plasma clean, holes, etc.
      • wash flow cell with Eth and water
      • add ConA for at least 5 mins
      • measure OD of cells
      • add cells and allow to sit for 5 mins
    • Measurement Parameters
      • Times
        • 210
        • 180
        • 150
        • 120
        • 100
        • 90
        • 80
        • 60
        • 30
        • 10
  • Methods (specific)
    • Set1: 210 and 180
    • Set2: 150 and 120
    • run Set1Wt, Set1Mutant, Set2Wt, Set2 Mutant on one day
    • Set3: 100 and 90 and 80
    • Set4: 60 and 30 and 10
    • run Set3Wt, Set3Mutant, Set4Wt, Set4Mutant on another day
    • Never sleep
    • 5 oscillations per time ie for 60 secs run 11 minutes, start and end with off
    • Wait 20 minutes to allow cells to reset in between different times of the same set
    • I need to go through the timing, but i think i need 3 days to get through each mutant


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Bohao_Liu/Notebook/Research/2012/10/20&oldid=798807"