User:Benjamin Friedel/Notebook/CHEM 471/2016/04/12: Difference between revisions
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-standardized silver reaction protein concentrations (too much casein as a holdover from previous protocols, not necessary) | -standardized silver reaction protein concentrations (too much casein as a holdover from previous protocols, not necessary) | ||
## UV Vis and Fluorescence Spectra before and after | ## UV Vis and Fluorescence Spectra before and after | ||
==Materials== | ==Materials== |
Revision as of 12:58, 4 May 2016
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Objectives
-standardized silver reaction protein concentrations (too much casein as a holdover from previous protocols, not necessary)
Materials
Procedure
-Fluorescence excited at 280nm for tryptophan and tyrosine fluorescence before and after reaction -scanned emission spectra from 300-540nm ( to avoid excitation peaks at 280 and 570nm) Reaction run on p1 cycle of oven (4 hours at 80˚C reaction time)
Results
-only Casein-Gold worked, though it was slightly opalescent indicating the presence of some precipitate |