User:Benjamin Friedel/Notebook/CHEM 471/2015/10/14: Difference between revisions
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Latest revision as of 01:20, 27 September 2017
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ObjectiveThe objective for today is to measure the UV absorbance of lysozyme AuNP fibers over various incubation times with alpha chymotrypsin using a bradford assay. ProtocolProtease Preparation
Incubation Tube Preparation (Samples and Blanks)
DataFigure 1
Figure 2 above shows the absorbance data of the AuNP samples over alpha-chymotrypsin incubation corrected for blanks. Blank correction was performed by subtracting the values of the Bradford blank and isosbestic point corrected blank data from the Bradford blank and isosbestic point corrected sample (AuNP fiber tube) data. The increase in absorbance as incubation time increases suggests that as alpha-chymotrypsin digests AuNP fibers samples, peptides and small fiber particles are released into solution. |