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Objective
Todays objective is to prepare gold and silver nanoparticles using pure casein and soy protein isolate as capping and reducing agents
Materials
Stock Solutions
- SPI (ARCON S 066400)
- Casein (amresco E666-500G)
- note store in refrigerator
- Fructose
- Choloroauric Acid (REM Chemicals)
- note store in desiccator, sensitive to metals
- Silver Nitrate (CMI 7985)
- note store in desiccator, sensitive to light
Procedure
Procedure to make the gold nanoparticles was based off of Dr. Hartings' notebook. Instead of lysozyme, Casein (an estimated MW at 22kD) was used. Then the concentration of Casein for 20uM was converted to g/ml, and this concentration was applied to the soy protein isolate (SPI) which does not have a clear MW.
The reaction spreadsheet is shown below. After creating the reaction mixes, samples were heated at 80˚C for 4 hours in the HERATHERM oven in Dr. Harting's Lab in Beeghly (on AU campus). This reaction cycle also consisted of 30 minutes of heating and cooling beforehand. Samples were retrieved the next day (1/27/16), checked for color change and then covered in tin foil completely (to prevent oxidation by light of potentially unreacted silver nitrate).
| 1/27/2016
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| 1mL total reaction |
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2.5mM HAuCl4 |
2.5mMAgNO3 |
.44mg/ml SPI |
.44mg/ml Casein |
2.5mM Fructose |
Water |
Au/Ag:Protein |
Notes
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1 |
40µL |
0 |
0 |
560µL |
0 |
400µL |
1:15 |
no color change
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2 |
40µL |
0 |
0 |
440µL |
0 |
520µL |
1:11 |
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3 |
40µL |
0 |
0 |
360µL |
0 |
600µL |
1:09 |
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4 |
40µL |
0 |
0 |
280µL |
0 |
680µL |
1:07 |
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5 |
40µL |
0 |
0 |
200µL |
0 |
760µL |
1:05 |
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6 |
0 |
40µL |
0 |
560µL |
0uL |
400µL |
1:15 |
all gold/bright yellow except for #14 which was lighter
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7 |
0 |
40µL |
0 |
560µL |
20uL |
380µL |
1:15 |
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8 |
0 |
40µL |
0 |
560µL |
40uL |
360µL |
1:15 |
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9 |
0 |
40µL |
0 |
360uL |
0uL |
600µL |
1:09 |
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10 |
0 |
40µL |
0 |
360µL |
20uL |
580µL |
1:09 |
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11 |
0 |
40µL |
0 |
360µL |
40uL |
560µL |
1:09 |
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12 |
0 |
40µL |
0 |
200uL |
0uL |
760µL |
1:05 |
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13 |
0 |
40µL |
0 |
200µL |
20uL |
740µL |
1:05 |
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14 |
0 |
40µL |
0 |
200µL |
40uL |
720µL |
1:05 |
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15 |
40µL |
0 |
560µL |
0 |
0 |
400µL |
1:15 |
Made fibers so need higher concentration of protein
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16 |
40µL |
0 |
440µL |
0 |
0 |
520µL |
1:11 |
purple fibers at bottom
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17 |
40µL |
0 |
360µL |
0 |
0 |
600µL |
1:09 |
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18 |
40µL |
0 |
280µL |
0 |
0 |
680µL |
1:07 |
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19 |
40µL |
0 |
200µL |
0 |
0 |
760µL |
1:05 |
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20 |
0 |
40µL |
560µL |
0 |
0uL |
400µL |
1:15 |
No color change
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21 |
0 |
40µL |
560uL |
0 |
20uL |
380µL |
1:15 |
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22 |
0 |
40µL |
560µL |
0 |
40uL |
360µL |
1:15 |
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23 |
0 |
40µL |
360uL |
0 |
0uL |
600µL |
1:09 |
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24 |
0 |
40µL |
360µL |
0 |
20uL |
580µL |
1:09 |
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25 |
0 |
40µL |
360µL |
0 |
40uL |
560µL |
1:09 |
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26 |
0 |
40µL |
200uL |
0 |
0uL |
760µL |
1:05 |
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27 |
0 |
40µL |
200µL |
0 |
20uL |
740µL |
1:05 |
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28 |
0 |
40µL |
200µL |
0 |
40uL |
720µL |
1:05 |
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| Controls |
29 |
40µL |
0 |
0 |
0 |
0 |
960µL |
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No color change
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30 |
0 |
40µL |
0 |
0 |
0 |
960µL |
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-oven started 4:35pm 1/27, removed and covered 10:30am 1/28
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Results
- Silver nanoparticles with casein were created as seen by a color change from clear to yellow, as well as a clear and solitary peak at 420nm in UV Vis.
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Project name
