User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/11/13: Difference between revisions
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| 11/10/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||[[Image: 20131110 122904 (3).jpg |250px]]||Amplified with V0200 Sequencing Primers to make sure Kozak-HSV-CFP-NLS-Stop-PolyA (2K bps are there). | | 11/10/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)]||[[Image: 20131110 122904 (3).jpg |250px]]||Amplified with V0200 Sequencing Primers to make sure Kozak-HSV-CFP-NLS-Stop-PolyA (2K bps are there). | ||
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| 11/11/2013||Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 72C (6 min.)]||||Column 1,2: 9 ng Plasmid, Column 3,4: 0.09 ng Plasmid, Column 5,6: 0.5 ml primer from 10mM solution | | 11/11/2013||Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 72C (6 min.)]||[[Image:20131110 213024 (2).jpg |200px]]||Column 1,2: 9 ng Plasmid, Column 3,4: 0.09 ng Plasmid, Column 5,6: 0.5 ml primer from 10mM solution | ||
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| 11/11/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 75C (6 min.)]||||Increase the extention temperature to 75C and annealing temperature to 49C | | 11/11/2013||0.09ng Plasmid: BD002||[95C (30 sec.), 49C (45 Sec.), 75C (6 min.)]||||Increase the extention temperature to 75C and annealing temperature to 49C |
Revision as of 13:35, 13 November 2013
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DateTroubleshooting BD002 PCR Linearization for GG Assembly
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