User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/31: Difference between revisions
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| colspan="8"| '''Incubate at room temperature for 30 minutes.''' | | colspan="8"| '''Incubate at room temperature for 30 minutes.''' | ||
|- | |- | ||
| '''Transformation result''' || <span style="color:#FF0000">6 Colonies</span> | | '''Transformation result''' || <span style="color:#FF0000">6 Colonies</span>|| <span style="color:#FF0000">12 Colonies</span>|| <span style="color:#FF0000">100s of Colonies and Satellite colonies</span> || <span style="color:#FF0000">19 Colonies</span> || <span style="color:#FF0000">100s Colonies and Satellite Colonies</span>|| <span style="color:#FF0000">100s Colonies and Satellite Colonies</span> || <span style="color:#FF0000">No Colony</span> | ||
|} | |} | ||
* Fast transformation protocol in DH5α-T. Add 30 μL of cells to each sample and incubate on ice for 30 minutes. | * Fast transformation protocol in DH5α-T. Add 30 μL of cells to each sample and incubate on ice for 30 minutes. | ||
* Grow on AMP agar plates and incubate overnight at 37°C. | * Grow on AMP agar plates and incubate overnight at 37°C. | ||
* Take 3 colonies from 1 and 2 and 4 colonies from plate 4 for Colony PCR using mutagenesis primers for linearization of the plasmids. |
Revision as of 12:37, 1 November 2013
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