User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/31: Difference between revisions
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| Total || 10.0 μl || 10.0 μl || 12.0 μl || 10.0 μl || 10.0 μl || 12.00 μL || 10.00 μL | | Total || 10.0 μl || 10.0 μl || 12.0 μl || 10.0 μl || 10.0 μl || 12.00 μL || 10.00 μL | ||
|- | |- | ||
| Incubate at room temperature for 30 minutes. | | colspan="8"| '''Incubate at room temperature for 30 minutes.''' | ||
|- | |- | ||
| '''Transformation result''' || || || || <span style="color:#FF0000">---</span>|| <span style="color:#FF0000">---</span> | | '''Transformation result''' || || || || <span style="color:#FF0000">---</span>|| <span style="color:#FF0000">---</span> || || | ||
|} | |} | ||
* Fast transformation protocol in DH5α-T. Add 30 μL of cells to each sample and incubate on ice for 30 minutes. | * Fast transformation protocol in DH5α-T. Add 30 μL of cells to each sample and incubate on ice for 30 minutes. | ||
* Grow on AMP agar plates and incubate overnight at 37°C. | * Grow on AMP agar plates and incubate overnight at 37°C. |
Revision as of 17:21, 31 October 2013
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