User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/29: Difference between revisions
From OpenWetWare
Line 35: | Line 35: | ||
* 60°C, 10 min. | * 60°C, 10 min. | ||
* 80°C, 20 min. | * 80°C, 20 min. | ||
* Decrease the temperature stepwise from 80°C to 4°C | |||
*4°C, ∞ | *4°C, ∞ | ||
|- | |- | ||
Line 49: | Line 50: | ||
|Result (Colonies) || 8 || NO || ~50 || ~80 || ~100 | |Result (Colonies) || 8 || NO || ~50 || ~80 || ~100 | ||
|} | |} | ||
* Bacterial transformation , Fast transformation protocol | * Bacterial transformation , Fast transformation protocol |
Revision as of 15:13, 30 October 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||
Golden Gate Assembly of BD002 to Replace HsvtkTATA with CMV and HPK Promoters (Third Try)
Backbone vector [math]\displaystyle{ X= (4600 / 209 ) * 0.013 * 20 = 5.72 }[/math] HPK [math]\displaystyle{ X= (516 / 70 ) * 0.013 * 20 = 1.91 }[/math], [math]\displaystyle{ 1.91 + 18.1 = 20 }[/math] CMV [math]\displaystyle{ X= (588 / 42 ) * 0.013 * 20 = 3.64 }[/math], [math]\displaystyle{ 3.64 + 16.36 = 20 }[/math]
|