User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/17: Difference between revisions
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== Golden Gate Assembly of BD002 to Replace HsvtkTATA with CMV and HPK Promoters== | |||
Desired Constructs: | |||
BD003: 5XGAL4-Spacer-'''CMV'''-Kozak-AMCyan-AmCyan-NLS-Stop | |||
BD004: 5XGAL4-Spacer-'''HPK'''-Kozak-AMCyan-AmCyan-NLS-Stop | |||
Existing construct is: 5XGAL4-Spacer-'''HsvTK'''-Kozak-AMCyan-AmCyan-NLS-Stop in V0200 [http://parts.igem.org/Part:BBa_J176121?title=Part:BBa_J176121 mammalian vector] | |||
Revision as of 14:54, 24 October 2013
 PcTF Subcloning in E. coli
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DateGolden Gate Assembly of BD002 to Replace HsvtkTATA with CMV and HPK PromotersDesired Constructs: BD003: 5XGAL4-Spacer-CMV-Kozak-AMCyan-AmCyan-NLS-Stop BD004: 5XGAL4-Spacer-HPK-Kozak-AMCyan-AmCyan-NLS-Stop Existing construct is: 5XGAL4-Spacer-HsvTK-Kozak-AMCyan-AmCyan-NLS-Stop in V0200 mammalian vector |
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