User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/10: Difference between revisions
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* Miniprep the plasmids, final concentration: '''491ng/μL''' | * Miniprep the plasmids, final concentration: '''491ng/μL''' | ||
[[Image:BD002-No BSMBI-Gel Proof.png|thumb| | [[Image:BD002-No BSMBI-Gel Proof.png|thumb|300px| Column 1 and 4 look the same, which shows that no BSMBI cut site is recognized in column 1, Colom 5 and 3 look the same which shows only SpeI works and linearizes the plasmid in column 5. Linearize plasmid size: ~6300 bps]] | ||
{| {{table}} ; style="text-align:center; width:550px; height:200px;" | {| {{table}} ; style="text-align:center; width:550px; height:200px;" | ||
| Plasmid DNA (BD002)|| 1 (4.0 μl) || 2 (4.0 μl)|| 3 (4.0 μl)|| 4 (4.0 μl)|| 5 (4.0 μl) | | Plasmid DNA (BD002)|| 1 (4.0 μl) || 2 (4.0 μl)|| 3 (4.0 μl)|| 4 (4.0 μl)|| 5 (4.0 μl) | ||
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# Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder. | # Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder. | ||
* '''Sequencing Analysis''' | |||
Mutated cut site is marked with red. | |||
[[Image:Sequencing Result.png | 600px]] |
Revision as of 14:03, 4 November 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10/10/12Site Directed Mutagenesis-Removing the BSMBI cut site from BD002 reporter gene
Mutagenesis Confirmation
Mutated cut site is marked with red. |