User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/10: Difference between revisions
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| DNA μL || 1μL || 2μL || 3μL || 5μL || 5μL | | DNA μL || 1μL || 2μL || 3μL || 5μL || 5μL | ||
|- | |- | ||
| T4 Ligase || 1.0 μl || 1.0μL | | T4 Ligase || 1.0 μl || 1.0μL || 1.0μL || 1.0μL || 0.0μL | ||
|- | |- | ||
| 2X Roche Buffer|| 5.00 μl || 5.00 μL || 5.00 μL || 5.00 μL || 5.00 μL | | 2X Roche Buffer|| 5.00 μl || 5.00 μL || 5.00 μL || 5.00 μL || 5.00 μL | ||
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| Incubate at room temperature for 30 minutes. | | Incubate at room temperature for 30 minutes. | ||
|} | |} | ||
* Long transformation protocol in BL-21. |
Revision as of 18:11, 13 October 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10/10/12Site Directed Mutagenesis-Removing the BSMBI cut site from BD002 reporter gene
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