User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/10: Difference between revisions
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[[Image:BD002-SD-SAPI.png |frame|none|alt=Alt text|Columns 1,3 amplified show BD002 ~6300 bp.]] | [[Image:BD002-SD-SAPI.png |frame|none|alt=Alt text|Columns 1,3 amplified show BD002 ~6300 bp.]] | ||
* Using the GenElute™ PCR Clean-Up Kit for rapid purification of double-stranded PCR amplification products. | * '''DNA Purification''' Using the GenElute™ PCR Clean-Up Kit for rapid purification of double-stranded PCR amplification products. | ||
** The purified result: Column1: 100ng/μL and Column 3: 28ng/μL. | ** The purified result: Column1: 100ng/μL and Column 3: 28ng/μL. | ||
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|- | |- | ||
| SAPI || 1.0 μl || 1.0 μL | | SAPI || 1.0 μl || 1.0 μL | ||
|- | |- | ||
| 10x buffer || 5.00 μl || 5.00 μL | | 10x buffer || 5.00 μl || 5.00 μL | ||
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* Incubate | * '''Restriction enzyme deactivation''' Incubate the tubes at 80°C for 10 minutes and cool down gradually in room temperature. | ||
'''Ligate The Sticky Ends to Make Circular DNA''' |
Revision as of 18:02, 13 October 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10/10/12Site Directed Mutagenesis-Removing the BSMBI cut site from BD002 reporter gene
Ligate The Sticky Ends to Make Circular DNA |