User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/09/07: Difference between revisions
From OpenWetWare
(→Date) |
|||
(One intermediate revision by the same user not shown) | |||
Line 9: | Line 9: | ||
=09/06/2013= | =09/06/2013= | ||
'''Confirm The | '''Confirm The Mutagenesis''' | ||
{| {{table}} cellspacing="3" width=800px | {| {{table}} cellspacing="3" width=800px | ||
Line 16: | Line 16: | ||
| Plasmid DNA || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl | | Plasmid DNA || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl || 3.0 μl | ||
|- | |- | ||
| | | BsmBI || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl | ||
|- | |- | ||
| Spe1 || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl | | Spe1 || 0.0 μl || 1.0 μl || 0.0 μl || 0.0 μl || 1.0 μl || 0.0 μl | ||
Line 28: | Line 28: | ||
| Incubate at 37°C for 10 minutes. | | Incubate at 37°C for 10 minutes. | ||
|} | |} | ||
[[Image:Ste-Directed-Test.jpg]] | |||
# Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder. | # Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder. | ||
* The gel picture shows that the BSMBI cut site did not remove. |
Revision as of 08:59, 13 October 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||
09/06/2013Confirm The Mutagenesis
|