User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/09/05: Difference between revisions
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* DpnI Digest (gets rid of methylated template DNA) | * DpnI Digest (gets rid of methylated template DNA) | ||
* Add 1 μL DpnI enzyme to the reaction. | |||
* Gently and thoroughly mix the reaction, spin down in a microcentrifuge for 1 minute, and immediately incubate at 37°C for 1 hour to digest the parental supercoiled dsDNA | |||
* Add 1 μL DpnI enzyme to | |||
* Gently and thoroughly mix | |||
* Transform 10 μl of the Dpn I-treated DNA from each control and sample reaction into separate 50-μl aliquots of BL21 cells | * Transform 10 μl of the Dpn I-treated DNA from each control and sample reaction into separate 50-μl aliquots of BL21 cells | ||
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Revision as of 15:59, 5 September 2013
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September 5, 2013
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