User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/09

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''Flourescent Microscopy'''
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'''Flourescent Microscopy'''
# Check RFP/CFP of [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/08 co-transfection] after 18 hrs.
# Check RFP/CFP of [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/08 co-transfection] after 18 hrs.
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| '''BioBrick Part''' || '''Vector''' || '''Description''' || Microscopy Image
| '''BioBrick Part''' || '''Vector''' || '''Description''' || Microscopy Image
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|-
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| KAH59 || PcVN || [K : Gal4 DB : mCh : mCh] + VP64-NLS || [[Image:Merge-KAH59-PcVN.png |320px]]
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| KAH59 || PcVN || [K : Gal4 DB : mCh : mCh] + VP64-NLS || [[Image:Merge-KAH59-PcVN.png |250px]]
|-
|-
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| KAH60 || PcVN || [K : Gal4 DB : mCh] + VP64-NLS || [[Image:Merge-KAH60-PcVN.png |320px]]
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| KAH60 || PcVN || [K : Gal4 DB : mCh] + VP64-NLS || [[Image:Merge-KAH60-PcVN.png |250px]]
|-
|-
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| KAH54 || PcVN || [[K : Gal4 DB] + VP64-NLS || [[Image:KAH54-PcVN.png |320px]]
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| KAH54 || PcVN || [[K : Gal4 DB] + VP64-NLS || [[Image:KAH54-PcVN.png |250px]]
|-
|-
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| KAH59 || PcN (MV5) || [K : Gal4 DB : mCh : mCh] + NLS || [[Image:Merged-KAH59(MV5).png |320px]]
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| KAH59 || PcN (MV5) || [K : Gal4 DB : mCh : mCh] + NLS || [[Image:Merged-KAH59(MV5).png |250px]]
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|-
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| KAH66 || PcN (MV5) || [K : Gal4 DB : mCh : mCh : VP64] + NLS || [[Image:Merged-KAH66-MV5.png |320px]]
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| KAH66 || PcN (MV5) || [K : Gal4 DB : mCh : mCh : VP64] + NLS || [[Image:Merged-KAH66-MV5.png |250px]]
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|-
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| KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector ||
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|}
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Revision as of 18:53, 9 August 2013

PcTF Subcloning in E. coli Main project page
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08/09/2013

Flourescent Microscopy

  1. Check RFP/CFP of co-transfection after 18 hrs.


BioBrick Part Vector Description Microscopy Image
KAH59 PcVN [K : Gal4 DB : mCh : mCh] + VP64-NLS
KAH60 PcVN [K : Gal4 DB : mCh] + VP64-NLS
KAH54 PcVN [[K : Gal4 DB] + VP64-NLS
KAH59 PcN (MV5) [K : Gal4 DB : mCh : mCh] + NLS
KAH66 PcN (MV5) [K : Gal4 DB : mCh : mCh : VP64] + NLS
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